MOLECULAR HISTOPATHOLOGY OF NEOPLASMS

Teaching her lesson "MOLECULAR HISTOPATHOLOGY OF NEOPLASMS" consists of lectures, tutorials and laboratory exercises. Attendance at exercises is mandatory.

The elections develop the material described above.

The tutorials (in 1-2 groups of students, 1 teacher per group) summarize and deepen the material and the basic principles of the methodologies used in Molecular Histopathology.

The laboratory exercises (in 1-2 groups of students, 1 teacher per group of 8-10 students) are the practical exercise of the students, they are a necessary supplement to the lectures and aim to familiarize them with the use of techniques, the operation of instruments and the conduct of frequently used tests in Molecular Histopathology of Neoplasms, as well as to help understand concepts that are not easily understood theoretically (learning based on practical experience).

Information and Communication Technologies are used to prepare lecture materials and provide online information and learning aids to students.

Specifically:

• Common software (eg powerpoint) is used to prepare lecture material and display slides and videos.
• The study guide (analytical material & additional bibliography), tutorial material, theory and laboratory exercise protocols, handover slides after each lesson and scientific articles related to the course material are made available to students electronically and online through e-class.
• Information about the course, the lecturers and their research interests and the Laboratory of Pathological Anatomy in general is available online on the website of the Department of Medicine of the Faculty of Medicine.

• Common software (eg excel) is used for the statistical processing of the student evaluation.
• Announcements, information, etc. are available online and through e-class. Communication is also done via e-mail.

Evaluation methods.

A. In the laboratory exercises: Work, Written Exams with short answer and problem solving questions.

Students' participation in the exercises is mandatory.

Successful examination of Laboratory after a work presentation.

B. In the tutorials: 

The material of the tutorials is examined in writing together with the material of the lectures.

C. In theory – lecture material: Written Exams with multiple choice and True/False questions, development questions, oral examination of students with special needs.

The course exams are written, lasting 2 hours, and consist of multiple choice or True/False questions and critical or short answer questions. Examinable material is the material of lectures and tutorials as described above.

Final grade

The final grade of the course is calculated by 60% from the grade of the written exams of the course and by 40% from the laboratory exercise-work.

All of the above can be accessed by students as they are posted electronically in the e-class.

The course material aims to understand the relationship between the molecular profile of neoplasms (genotype) and the morphological characteristics (phenotype) in the human body. It also refers to applications of the molecular profile of tumors in clinical practice, i.e. the diagnosis and treatment of the most common solid neoplasms based on the molecular characteristics of the tumor.

Finally, the aim of the course is for students to gain a thorough understanding of the diagnostic approach to neoplasms and the necessity of molecular markers with prognostic and predictive character for the correct and effective therapeutic approach to patients.

Upon successful completion of the course, the student will be able to:

• He has understood the basic elements of carcinogenesis and their relationship with the morphological characteristics of neoplastic cells in the human body.
• He has knowledge of the basic genetic changes and the mechanisms that lead to neoplastic transformation.
• Is able to use and understand basic terms of molecular histopathology.
• Uses the basic equipment of a molecular histopathology laboratory and performs basic molecular analyses.
• Analyzes and understands the action of the main signaling pathways involved in carcinogenesis in the human body.
• Can collaborate with fellow students in a laboratory environment to perform basic laboratory techniques used in molecular histopathology.

General Skills

• Search, analysis and synthesis of data and information, using the necessary technologies
• Adaptation to new situations
• Decision making
• Autonomous work
• Teamwork
• Exercise criticism and self-criticism
• Promotion of free, creative and inductive thinking

A. Theoretical clinical skills
I. By theory chapter
1. The nature of the Cancer cell – Biological characteristics of cancer cells
2. Maintenance of genomic integrity and cancer generation
3. Uncontrolled cell proliferation and tumorigenesis
4. Growth factors, growth factor receptors and histogenesis – Primordial progenitor cells (Stem Cells)
5. Extracellular – intracellular signaling
6. Transcription factors – regulation of transcription
7. Oncogenes – Tumor suppressor genes
8. Mechanisms of infiltration - metastasis - EMT
9. Biology of Angiogenesis
10. Mechanisms of carcinogenesis – Biomarkers – Biological treatments in frequent neoplasms (sporadic forms)
11. Mechanisms of carcinogenesis – Biomarkers – Biological treatments in frequent neoplasms (familial forms)
13. Molecular methods of diagnostic approach to neoplasms in solid tumors
14. Molecular methods of diagnostic approach to neoplasms in liquid biopsies

II. In the compulsory tutoring schools
Theoretical background of basic laboratory techniques.

B. Practical and clinical skills
In the mandatory workshops – learning based on practical experience
Acquisition of general and specific practical skills by the medical students during the preparation
of laboratory exercises:
1. General skills
• observation of work flow in the laboratory
• use of standard laboratory techniques
• effective team work
• safe work in a laboratory environment
• receiving and following instructions
• perception of laboratory errors and identification of their sources
2. Special skills
Familiarity with basic laboratory molecular techniques

C. Knowledge – Lecture material
1. The nature of the Cancer cell – Biological characteristics of cancer cells (clonality, autonomy, loss of differentiation)
2. Maintenance of genomic integrity and cancer generation
3. Uncontrolled cell proliferation and tumorigenesis-apoptosis
4. Growth factors, growth factor receptors and histogenesis – Primordial progenitor cells (Stem Cells)
5. Extracellular – intracellular signaling
6. Transcription factors – regulation of transcription
7. Oncogenes – Tumor suppressor genes
8. Mechanisms of infiltration - metastasis - EMT
9. Biology of Angiogenesis
10. Mechanisms of carcinogenesis – Biomarkers – Biological treatments in frequent neoplasms (sporadic forms)
11. Mechanisms of carcinogenesis – Biomarkers – Biological treatments in frequent neoplasms (familial forms)
13. Molecular methods of diagnostic approach to neoplasms in solid tumors
14. Molecular methods of diagnostic approach to neoplasms in liquid biopsies

Recommended books:

1. Human Molecular Genetics

Edition: 1/2023

Authors: Strachan Tom, Read Andrew P.

Type: Journal

ISBN: 9789925350742

Code EVDOXOS: 112691149

Owner (Publisher): BROKEN HILL PUBLISHERS LTD 

2. The Cell

Edition: 8th/2021

Authors: Geoffrey M. Cooper

Type: Journal

ISBN: 9786185135201

Code EVDOXOS: 102123643

Owner (Publisher): ACADEMIC PUBLICATIONS I. BASDRA & SIA O.O. 

Other writings:

NO

B. LABORATORIES

NO

Related scientific journals:

YES